Journal: Polymers

Article Title: Laser Surface Microstructuring of a Bio-Resorbable Polymer to Anchor Stem Cells, Control Adipocyte Morphology, and Promote Osteogenesis

doi: 10.3390/polym10121337

Figure Lengend Snippet: Representative immunofluorescence images of MSCs on glass cover slips or on four different PLLA surfaces after 14 days in culture: ( a ) MSCs on cover slips; ( b ) MSCs on FLAT PLLA; ( c ) MSCs on ROUGH PLLA; ( d ) MSCs on GROOVES 1; ( e ) MSCs on GROOVES 2. Focal adhesions marked with Vinculin are shown in green, cell cytoplasm marked with Phalloidin in red and cell nuclei marked with DAPI in blue.

Article Snippet: Cell morphology was examined on all PLLA surfaces by immunofluorescence microscopy applying two different staining methods: cells were stained with NeuroDio, a green-fluorescent cytoplasmic membrane stain (Promokine, Promocell, Heidelberg, Germany) before seeding and, on the other hand, cells were fixed and stained for DAPI (Vector Biolabs, H-1200, Malvern, PA, USA) (cell nuclei, blue), phalloidin (Thermo Fisher Scientific, A12381, Darmstadt, Germany) (cell cytoplasm, red), and monoclonal anti-vinculin antibody (Sigma, V9131, Sigma-Aldrich, Munich, Germany) (focal adhesions, green).

Techniques: Immunofluorescence